Description
Coleção de vírus de CTV contendo estirpes potencialmente protetivas e estirpes fortes do Citrus tristeza virus do estado do Paraná.
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 35 records.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Molina R O, Souza C F (2022): Coleção de virus do IDR - Paraná. v1.0. No organisation. Dataset/Occurrence. https://ipt.sibbr.gov.br/taxonline/resource?r=idr_pr_virologia_01&v=1.0
Rights
Researchers should respect the following rights statement:
This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF Registration
This resource has not been registered with GBIF
Keywords
virus paraná ctv
Contacts
- Originator
https://orcid.org/0000-0002-6337-5235
- Point Of Contact
- Metadata Provider
- User ●
- Point Of Contact
- Point Of Contact
Geographic Coverage
Dados coletados no estado Paraná.
| Bounding Coordinates | South West [-27.255, -55.283], North East [-22.513, -47.549] |
|---|
Taxonomic Coverage
Vírus da tristeza dos citros
| Species | Citrus tristeza virus (Vírus da tristeza dos citros) |
|---|
Temporal Coverage
| Formation Period | 2021 |
|---|
Project Data
Gene P23 do Citrus tristeza virus de complexos potencialmente protetivos e de complexos fortes do vírus.
| Title | Coleção de Microorganismos IDR-PR - Virologia |
|---|---|
| Identifier | Coleção CTV P23 |
| Study Area Description | Fitopatologia |
The personnel involved in the project:
Sampling Methods
Extração de RNA total, RT-PCR seguido de clonagem e sequenciamento.
| Study Extent | Amostras de vírus retiradas de pomares comerciais do estado do Paraná |
|---|
Method step description:
- As amostras foram maceradas com nitrogênio líquido. O RNA total foi extraído com o reagente Trizol, seguindo instruções do fabricante. Realizado RT-PCR com primers pm85 e pm86. Clonagem com kit Topo Ta da Invitrogen conforme orientações do fabricante. Sequenciamento do gene P23 em empresa especializada.
Collection Data
| Collection Name | Coleção de virologia do IDR - PR I |
|---|
| Specimen preservation methods | Deep frozen |
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Additional Metadata
| Purpose | Publicar as coleções de microrganismos do IDR PR |
|---|---|
| Alternative Identifiers | https://ipt.sibbr.gov.br/taxonline/resource?r=idr_pr_virologia_01 |